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| Metabolomic Study of Biochemical Changes related to toxicity induced by bupleurotoxin Using LC-MSCoupled with a Pattern Recognition Approach |
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| DOI：10.15806/j.issn.2311-8571.2014.0008 |
| KeyWord:bupleurotoxin, metabolomic profiling, toxicity, biomarker, LC-Q-TOF-MS |
| Author | Institution |
| Zhong-xiao Zhang |
School of Pharmacy, Second Military Medical University, Shanghai, , P.R. China |
| Juan Su |
School of Pharmacy, Second Military Medical University, Shanghai, , P.R. China |
| Xin-ru Liu |
School of Pharmacy, Second Military Medical University, Shanghai, , P.R. China |
| Wei-xing Dai |
School of Pharmacy, Second Military Medical University, Shanghai, , P.R. China |
| Shi-kai Yan |
School of Pharmacy, Shanghai Jiao Tong University, Shanghai , P.R. China |
| Weidong Zhangab |
a.School of Pharmacy, Second Military Medical University, Shanghai, , P.R. China;b.School of Pharmacy, Shanghai Jiao Tong University, Shanghai , P.R. China |
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| Abstract: |
| The purpose of this study was to detect changes in urine of mice and to clarify the toxicity induced by bupleurotoxin (BETX) using liquid
chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). A procedure for urine analysis using pattern recognition was
proposed to evaluate the toxicity induced by BETX in male BALB/c mice. BETX at 2.5 mg/kg was administered intraperitoneally (i.p.), and
urine samples for the metabolomic study were collected from control and BETX experimental groups. Changes in the concentrations of some
urine metabolites were detected exclusively in the experimental group. All results suggested that exposure to BETX might cause a disturbance
in fatty acid metabolism and the oxidative stress system. These results may not only clarify the underlying mechanism of diverse intoxication
effects of BETX but also provide the guidance in preclinical toxicity screening for new drugs. |
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