Panax Notoginseng Saponins Restrains Ischemiareperfusion-induced Rat Mesenteric MicrocirculatoryDisturbance

Yu Zhang<sup>acd</sup>; Yu-Ying Liu<sup>bcd</sup>; Quan Li<sup>bcd</sup>; Chun-Shui Pan<sup>bcd</sup>; Jing-Yu Fan<sup>bcd</sup>; Chuan-She Wang<sup>abcd</sup>; Kai Sun<sup>abcd</sup>; Jing-Yan Han<sup>abcd*</sup>

Panax Notoginseng Saponins Restrains Ischemiareperfusion-induced Rat Mesenteric MicrocirculatoryDisturbance

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DOI：10.15806/j.issn.2311-8571.2014.0011

KeyWord:ICAM-1, Src, leukocytes adhesion, mast cell degranulation, panax notoginseng

Author	Institution
Yu Zhang^acd	a.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Yu-Ying Liu^bcd	b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Quan Li^bcd	b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Chun-Shui Pan^bcd	b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Jing-Yu Fan^bcd	b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Chuan-She Wang^abcd	a.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, , Beijing, China.b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Kai Sun^abcd	a.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, , Beijing, China.b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.
Jing-Yan Han^abcd*	a.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, , Beijing, China.b.Tasly Microcirculation Research Center, Peking University Health Science Center, , Beijing, China.c.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.d.Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People’s Republic of China, , Beijing, China.

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Abstract:

Objective: To investigate the effect of Panax notoginseng saponins (PNS) on ischemia reperfusion (I/R) induced rat mesenteric microcirculatory dysfunctions. Methods: Male Wistar rats weighting 200–250 g were subjected to 10 min ligation of the superior mesenteric artery and vein, followed by 60 min reperfusion. PNS (5 mg/kg/hr) was continuously administrated starting from 10 min before ischemia or 10 min after reperfusion until 60 min after reperfusion via left jugular vein. Leukocytes adhesion, mast cell degranulation, endothelial peroxidation, and albumin leakage of rat mesenteric venules were observed. Serum myeloperoxidase (MPO) level, intercellular adhesion molecule-1 (ICAM-1) expression and Src phosphorylation were examined. Results: PNS ameliorated leukocyte adhesion and mast cell degranulation, while with no obvious effects on endothelial peroxidation and albumin leakage. In addition, PNS inhibited serumMPO increase, intestinal ICAM-1 expression and Src phosphorylation induced by I/R. Conclusions: PNS ameliorated I/R-induced leukocyte adhesion and mast cell degranulation, the former is related to its inhibition of Src phosphorylation and ICAM-1 expression.