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| Paeonol improves lipopolysaccharide-inducedmicrocirculatory disturbance in rat mesentery |
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| DOI：10.15806/j.issn.2311-8571.2014.0019 |
| KeyWord:Paeonol, Microcirculation, Leukocyte adhesion, Albumin leakage |
| Author | Institution |
| Lei Dongac |
a.Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing , China;c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China |
| Ang Liac |
a.Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing , China;c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China |
| Mei-Li Duan |
Department of Critical Care Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing , China |
| Kai Sunbcde |
b.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing , China;c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China;
d.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing , China;e.Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing , China. |
| Yu-Ying Liucde |
c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China;d.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China,Beijing , China;e.Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing , China. |
| Ming-Xia Wangcde |
c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China;d.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China,Beijing , China;e.Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing , China. |
| Chuan-She Wangbcde |
b.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing , China;c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China;
d.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing , China;e.Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing , China. |
| Jing-Yu Fan |
Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing ,China |
| Jing-Yan Hanbcde* |
b.Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing , China;c.Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing , China;
d.Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of China, Beijing , China;e.Key Laboratory of Stasis and Phlegm of State Administration of Traditional Chinese Medicine, Beijing , China. |
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| Abstract: |
| Objective: To investigate the effect of paeonol on lipopolysaccheride (LPS)-induced rat mesenteric microcirculatory dysfunctions.Methods: Male Wistar rats were randomly distributed into 5 groups (n=6 in each): Sham group, LPS group, paeonol group, paeonol+LPS
group, and LPS+paeonol group. Endotoximia model was conducted by continuous LPS infusion. Changes in mesenteric microcirculatory
variables, including diameter of venule, velocity of red blood cells in venule, leukocyte adhesion, free radicals produced in venule and albumin leakage from venule, were observed through an inverted intravital microscope. Meanwhile, the expression of myeloperoxidase (MPO), CD18,intercellular adhesion molecule-1 (ICAM-1), toll-like receptor 4 (TLR4), nuclear factor-kappa B p65 subunit (NF-κB p65), activator protein-1
(AP-1), and Jun N-terminal kinase (JNK) was assessed by Western blot.Results: After infusion of LPS, the number of leukocytes adherent to venular wall, the intensity of dihydrorhodamine 123 (DHR)fluorescence in the venular walls, and albumin leakage from venules were significantly increased, whereas the red blood cell velocity in venule was decreased. All the manifestations were significantly reduced by pre-treatment and post-treatment with paeonol. Moreover, paeonol significantly attenuated the expression of MPO, CD18, ICAM-1, TLR4, NF-κB p65, AP-1 and JNK in rat mesentery after LPS.
Conclusions: The results demonstrated that paeonol could protect from and ameliorate the microcirculation disturbance induced by LPS |
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