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| Simultaneous Determination of Four Anthraquinones inPolygoni Multiflori Radix with Single Reference Standardby High Performance Liquid Chromatography |
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| DOI：10.15806/j.issn.2311-8571.2015.0021 |
| KeyWord:Polygoni Multiflori Radix, Single standard to determine multi-components method, Anthraquinone, Emodin |
| Author | Institution |
| Hua Yang |
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
| Jun-Fang Jia |
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
| Rui Wang |
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
| Fang Long |
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
| Ping Li* |
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
| Hui-Jun Li* |
State Key Laboratory of Natural Medicines,
China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing , China |
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| Abstract: |
| Objective: To establish a rapid, accurate and reliable analytical method for the simultaneous determination of four major anthraquinones in
Polygoni Multiflori Radix (PMR) using single reference standard.
Methods: The four components including emodin-8-O-β-D- (EMG), physcion-8-O-β-D-glucoside, emodin and physcion were separated on an
ODS C18 column within 13 min and detected at 280 nm. Emodin was selected as the reference standard, and the response factor for each
analyte with respect to emodin were calculated. Robustness were also tested including different columns, equipments, temperatures, detection
wavelengths, and other chromatographic conditions which might influence stability of response factors.
Results: The method was validated in terms of linearity (r2 > 0.9995), LOQs (0.820–3.05 ng/mL), LODs (0.180–0.920 ng/mL), precision,
accuracy (95.8–103.6%, RSD < 2.80%) and stability. A total of 40 batches of PMR were analyzed and the results were found to have no
statistically significant differences compared with those obtained using the external standard method.
Conclusion: This work provided a single standard to determine multi-components method for quantitation of four anthraquinones in PMR,
which could be applied in the quality control of this herbal drug. |
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