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| Chemical Characteristics Combined with Bioactivity forComprehensive Evaluation of Tumuxiang Based onHPLC-DAD and Multivariate Statistical Methods |
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| DOI：10.15806/j.issn.2311-8571.2015.0035 |
| KeyWord:Inula helenium L., Inula racemosa Hook f., HPLC fingerprints, Spectrum-efficacy relationship, Multivariate statistical methods |
| Author | Institution |
| Xia Gao |
School of Pharmacy, Lanzhou University, Lanzhou , China |
| Yu-Ling Ma |
School of Pharmacy, Lanzhou University, Lanzhou , China |
| Pei Zhang |
School of Pharmacy, Lanzhou University, Lanzhou , China |
| Xiao-Ping Zheng |
School of Pharmacy, Lanzhou University, Lanzhou , China |
| Bo-Lu Sun |
School of Pharmacy, Lanzhou University, Lanzhou , China |
| Fang-Di Hu* |
School of Pharmacy, Lanzhou University, Lanzhou Donggang Rd West199, Lanzhou, , China |
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| Abstract: |
| Background: The dried roots of Inula helenium L. (IH) and Inula racemosa Hook f. (IR) are used commonly as folk medicine under the
name of “tumuxiang (TMX)”. Phenolic acid compounds and their derivatives, as main active constituents in IH and IR, exhibit prominent
anti-inflammation effect.
Objective: To develop a holistic method based on chemical characteristic and anti-inflammation effect for systematically evaluating the
quality of twenty-seven TMX samples (including 18 IH samples and 9 IR samples) from different origins.
Methods: HPLC fingerprints data of AL (Aucklandia lappa Decne.) whose dried root was similar with HR was added for classification
analysis. The HPLC fingerprints of twenty-seven TMX samples and four AL samples were evaluated using hierarchical clustering analysis
(HCA) and principle component analysis (PCA). The spectrum-efficacy model between HPLC fingerprints and anti-inflammatory activities
was investigated by principal component regression (PCR) and partial least squares(PLS).
Results: All samples were successfully divided into three main clusters and peaks 7, 9, 11, 22, 24 and 26 had a primary contribution to classify
these medicinal herbs. The results were in accord with the appraisal results of herbs. The spectrum-efficacy relationship results indicated that
citric acid, quinic acid, caffeic acid-β-D-glucopyranoside, chlorogenic acid, caffeic acid, 1,3-O-dicaffeoyl quinic acid, tianshic acid and
3β-Hydroxypterondontic acid had main contribution to anti-inflammatory activities.
Conclusion: This comprehensive strategy was successfully used for identification of IH, IR and AL, which provided a reliable and adequate
theoretical basis for the bioactivity relevant quality standards and studying the material basis of anti-inflammatory effect of TMX. |
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