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| Identification of Common Edible and MedicinalMushrooms by DNA Barcoding |
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| DOI：10.15806/j.issn.2311-8571.2016.0016 |
| KeyWord:Edible mushroom, Medicinal mushroom, ITS, DNA Barcoding |
| Author | Institution |
| Yan-Yan Suab |
a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China
b.Huaibei Normal University, Huaibei , China |
| Huan Tanga |
a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China |
| Xi-Wen Lia |
a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China |
| Wei Suna |
a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China |
| Zeng-Hua Chenc |
c.Beijing Qianjunfang Academy of Sciences, Beijing , China |
| Li Xianga* |
a.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing , China |
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| Abstract: |
| Objective: To identify common edible and medicinal mushrooms using the internal transcribed spacer (ITS) region.
Method: A total of eighty-five samples belonging to forty-three species were used in this study. ITS regions were amplified and sequenced.
All ITS regions were analyzed using BLAST and MEGA 5.10 methods.
Results: Forty species were identified correctly via BLAST method whereas forty-one species were distinguished via Neighbor joining (NJ)
tree. All the species could be distinguished by BLAST combined with NJ tree and ITS sequence characteristics.
Conclusion: Forty-three common species of edible and medicinal mushrooms were effectively identified using DNA barcoding technology
based on ITS region |
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