Identification of Dian Ji Xue Teng (Kadsura interior) withDNA barcodes

Hao-Jie Guo<sup>a</sup>; Xi-Wen Li<sup>b</sup>; Yao-Dong Qi<sup>a</sup>; Xue-Ping Wei<sup>a</sup>; Ben-Gang Zhang<sup>a</sup>; Hai-Tao Liu<sup>a*</sup>

Identification of Dian Ji Xue Teng (Kadsura interior) withDNA barcodes

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DOI：10.15806/j.issn.2311-8571.2016.0017

KeyWord:DNA barcoding, Schisandraceae, SNP, Kadsura interior

Author	Institution
Hao-Jie Guo^a	a.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Xi-Wen Li^b	b.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China
Yao-Dong Qi^a	a.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Xue-Ping Wei^a	a.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Ben-Gang Zhang^a	a.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
Hai-Tao Liu^a*	a.Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China

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Abstract:

Objective: To identify Kadsura interior (Dian Ji Xue Teng, Schisandraceae) by using DNA barcoding. Methods: We analyzed five DNA barcodes (ITS, ITS2, psbA-trnH, matK and rbcL) using DNA barcoding in terms of distance-based, tree-based and character-based identification to distinguish Kadsura interior and its adulterants. Results: In distance-based and tree-based identification, K. interior could be distinguished easily from the species of Schisandra and K. coccinea. In character-based identification, there are two single nucleotide polymorphisms (SNPs) in ITS and one SNP in psbA-trnH which can be used to distinguish K. interior from K. heteroclita and K. longipedunculata. Conclusion: The results indicate that DNA barcoding can be used to identify K. interior. ITS and psbA-trnH sequence can be the most ideal DNA barcode for discriminating K. interior and its adulterants by the combination analysis of distance-based, tree-based and character-based identification (SNPs).