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| Monoclonal Antibody Usage Strategies for Natural Products inTraditional Chinese Medicine |
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| DOI：10.4103/wjtcm.wjtcm_5_17 |
| KeyWord:Eastern blotting, immunoaffinity concentration, Knockout extract, monoclonal antibody, single‑chain variable fragment |
| Author | Institution |
| Yukihiro Shoyamaa |
a.Department of Pharmacognosy, Faculty of Pharmaceutical Science, Nagasaki International University, Sasebo, Nagasaki 859‑3298, Japan |
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| Hits: 1422 |
| Download times: 2005 |
| Abstract: |
| Monoclonal antibodies (MAbs) against natural products with low‑molecular weights have become an important tool when combined with
other analytical systems. Eastern blotting involves a typical staining system wherein, for example, glycosides can be blotted to a membrane
and cross‑linked and stained using MAbs. An immunoaffinity column combined with a monoclonal antibody allows a one‑step purification
of hapten compounds or preparation of a knockout extract that removes only the target hapten molecules from a crude extract. Here, we
discuss the application of these extracts. Single‑chain variable fragment (scFv) proteins have led to novel assay systems such as fluobodies or
antibodies coupled with green fluorescent protein for natural products. A typical novel application of a scFv gene would be in the context of
plant breeding; this is designated “missile‑type” molecular breeding, intended to increase hapten molecule concentrations in transgenic plants |
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