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| Comparative analysis of four active compounds ofBaikal skullcap and its classical TCM prescriptionsaccording to different clinical curative effects |
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| DOI：10.15806/j.issn.2311-8571.2017.0001 |
| KeyWord:Baikal skullcap, Couplet medicine, Clinical curative effect, Compatibility of medicines, Proportions of the chemical compounds |
| Author | Institution |
| Guang-Wei Zhua* |
a Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, , China |
| Sheng-Jun Mab |
b.School of Food Sciences and Pharmacy, Xinjiang Agriculture University, Urumqi, , China |
| Ao-Xue Liuc |
c.School of Chinese Materia, Beijing University of Chinese Medicine, Beijing, , China |
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| Abstract: |
| Objective: A sensitive HPLC-DAD detection method was established for the comparative analysis of the four active compounds (including
baicalin, baicalein, wogonoside and wogonin) of Baikal Skullcap and its classical TCM prescriptions according to different clinical curative
effects. And analyze the relationship between compatibility of medicines, content and clinical curative effect.
Methods: Water extracts were analyzed by high-performance liquid chromatography-diode array detection (HPLC-DAD), using an Agilent
extend-C18 (4.6mm×250mm,5μm) column by using solvent A (0.2% phosphoric acid water) and solvent B (methanol) in the ratio of 52:48
(v/v) at a flow rate of 1 ml/min. The column temperature was maintained at 25°C and the detection wavelength was set at 280 nm.
Results: In quantitative analysis, the four selected markers showed good regression (R2>0.999) within test ranges. The average recoveries
were between 99.75~100.60% and their RSD values were between 0.97%~1.71%. The proportions of the four chemical compounds for different
efficacy were 33.51~35.76: 6.78~6.87: 3.45~3.64: 1; 18.6~23.64: 3.63~4.27: 1.36~1.53: 1; 29.94~31.78: 5.14~5.23: 3.28~3.56: 1; 33.99~41.91:
5.83~7.42: 2.83~3.39: 1.
Conclusion: Clinical practice of thousands of years have proved that the curative effect of Traditional Chinese Medicine depends on the
compatibility of different kinds of medicine material crude slices. According to this study we found that different compatibility of medicines
leads to different proportions of the chemical compounds. We assume that there is a certain correlation between therapeutical effect of TCM
and compatibility of medicines. |
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