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| Effects of Peiyuan Tongnao Capsule on Working Memory and the Expression of Glutamic Acid and Receptor in Hippocampal Area in Rats with Cerebral Ischemia |
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| DOI：10.4103/wjtcm.wjtcm_8_19 |
| KeyWord:Cerebral ischemia, Glu, NMDA receptor 2B, Peiyuan Tongnao capsule, working memory |
| Author | Institution |
| Jing Bai a |
a.Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine |
| Ying Gaoab |
a.Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine;b Institute of Encephalopathy, Dongzhimen Hospital,Beijing University of Chinese Medicine, Beijing, China |
| Yong-Hong Gaob |
b.Institute of Encephalopathy, Dongzhimen Hospital,Beijing University of Chinese Medicine, Beijing, China |
| Wang Lia |
a.Department of Neurology, Dongzhimen Hospital, Beijing University of Chinese Medicine |
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| Abstract: |
| Objective: To explore the effects of Peiyuan Tongnao (PYTN) Capsule on working memory, the content of glutamic acid, and the expression
of NMDA receptor 2B (NR2B) in rats with cerebral ischemia. Materials and Methods: 52 Sprague-Dawley rats were randomly divided
into sham group, model group, nimodipine group, and PYTN group. The bilateral common carotid artery occlusion (BCAO) was performed
to establish rat model with cerebral ischemia in the model, nimodipine, and PYTN groups. Gastric lavage with some drug based on body
weight conversion was performed daily for 4 weeks in the nimodipine and PYTN groups. The working memory of the rats was tested
by Morris water maze. The expression of Nissl body in hippocampus tissue was observed by Nissl staining. The determination of Glu
content in hippocampus was detected by high-performance liquid chromatography. The expression level of NR2B in hippocampus area
was determined using Western blot. Results: Morris water maze test on working memory escape latency. In the model group, day 1 versus
day 4, there was a statistical difference (P < 0.05). In the nimodipine group, day 1 vs. day 4 displayed a statistical difference (P < 0.05).
In the PYTN group, day 1 versus day 3 and day 1 versus day 4 were in significant differences (P < 0.05). The Glu content in hippocampus
of the sham group was significantly different from that of model group (P < 0.05). The Glu content in the PYTN group was significantly
different from that of the model group (P < 0.05). With regard to the expression of NMDA 2B in hippocampus between the sham group
and the model, nimodipine, and PYTN groups, all were displayed statistical significance (P < 0.01). As the same, the expression of NMDA
2B in the model group was significant from that of the nimodipine group (P < 0.05) and PYTN group (P < 0.01). Conclusion: PYTN
capsule was beneficial for improving working memory and protect neural cells in rats of cerebral ischemia, which may be associated with
upregulation of the expression of Glu and NMDAR2B in hippocampus. |
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