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| Exploring the Pharmacological Mechanism of Danhe Granules against Hyperlipidemia by Means of Network Pharmacology and Verified by Preliminary Experiments |
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| DOI：10.4103/wjtcm.wjtcm_59_21 |
| KeyWord:Component‑target network construction, Danhe granules, hyperlipidemia, network pharmacology, oxidative stress |
| Author | Institution |
| Zhi‑Qing Zhanga |
a.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China |
| Ai‑Ping Chena |
a.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China |
| Tong Yua |
a.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China |
| Shuang‑Jie Yanga |
a.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China |
| De‑Shuai Yub |
b.Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China |
| Ran Yangc |
c.Department of Cardiology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China |
| Xin‑Lou Chaia |
a.School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China |
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| Abstract: |
| Objective: Thisstudy explored the multicomponent, multitarget, and multipathway mechanism of Danhe granules(DG) against hyperlipidemia
through network pharmacology. The relevant targets and pathways were verified by preliminary experiments. Methods: The active components
of DG were selected by TCMSP and TCMIP database, and the component-target network diagram was constructed by Cytoscape 3.7.1. The
protein–protein interaction network of targets was constructed and core targets were screened out by STRING11.0 database. Metascape database
and Cytoscape 3.7.1 were used to enrich the target and establish a hyperlipidemia model in Sprague-Dawley (SD) rats to detect blood lipid
and oxidative stress indexes and observed pathological changes of aorta by H and E staining. Results: The results showed that a total of seven
active components of DG were screened out, including quercetin, sitosterol, luteolin, kaempferol, etc. There were 127 corresponding targets,
including AKT1, tumor necrosis factor, TP53, interleukin‑6, RELA, vascular endothelial growth factor, superoxide dismutases, and catalase.
It is mainly involved in biological processes such as drug response, regulation of apoptosis, redox reaction, and lipid reaction. There were
573 signal pathways corresponding to the target, including HIF-1 signal pathway, TNF signal pathway, VEGF signal pathway, nonalcoholic
fatty liver disease, etc. The experiment verified that DG can reduce the blood lipid of SD rats by regulating the process of oxidative stress.
Conclusions: This study made a preliminary study on the pharmacological mechanism of DG against hyperlipidemia and laid the foundation
for the research and development of new drugs and subsequent in-depth research. |
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